Team:Grinnell/Notebook/Gels/Promoters

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=Gels for Promoters=
=Gels for Promoters=
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==Dates==
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==2011.06.12-2011.06.18==
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<html>
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<table class="gallery" frame='void' rules='none'>
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<tr>
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<td>
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<a name="20110614_Promoters" href="https://2011.igem.org/File:20110614_Promoters.jpg">
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<img alt="20110614_Promoters" src="https://static.igem.org/mediawiki/2011/7/76/20110614_Promoters.jpg">
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</a>
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</td>
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<td>
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<a name="20110616_promotorGel" href="https://2011.igem.org/File:20110616_promotorGel.jpg">
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<img alt="20110616_promotorGel" src="https://static.igem.org/mediawiki/2011/9/9e/20110616_promotorGel.jpg">
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</a>
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</td>
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<td>
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<a name="20110616_promoterPostCleanupCrop" href="https://2011.igem.org/File:20110616_promoterPostCleanupCrop.jpg">
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<img alt="20110616_promoterPostCleanupCrop" src="https://static.igem.org/mediawiki/2011/f/f6/20110616_promoterPostCleanupCrop.jpg">
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</a>
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</td>
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</tr>
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<tr>
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<td>
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Figure 1: Promoters P<sub>xyl</sub> (inducible) and P<sub>rsaA</sub> (constitutive). Lane 1: ladder; lane 2: P<sub>rsaA</sub>; Lane 3: P<sub>xyl</sub>. Bands appear, but are hazy and spread out due to the small size of DNA fragments.
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</td>
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Figure 2: PCR products of <i>Caulobacter</i> promoters P<sub>rsaA</sub> and Pxyl.  Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>.
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Figure 3: Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>.
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</td>
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</tr>
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</table>
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</html>
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==2011.06.19-2011.06.25==
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<html>
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<table class="gallery" frame='void' rules='none'>
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<tr>
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<td>
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<a name='20110620_promotorsTransformationGel' href='https://2011.igem.org/File:20110620_promotorsTransformationGel.jpg'>
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<img alt='20110620_promotorsTransformationGel' src='https://static.igem.org/mediawiki/2011/4/4c/20110620_promotorsTransformationGel.jpg' />
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</a>
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</td>
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<td>
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<a name='20110621_BBaGel1' href='https://2011.igem.org/File:20110621_BBaGel1.jpg'>
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<img alt='20110621 BBaGel1' src='https://static.igem.org/mediawiki/2011/4/43/20110621_BBaGel1.jpg' />
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</a>
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</td>
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<td>
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<a name='20110622_PromotersGel2' href='https://2011.igem.org/File:20110622_PromotersGel2.jpg'>
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<img alt='20110622_PromotersGel2' src='https://static.igem.org/mediawiki/2011/a/a3/20110622_PromotersGel2.jpg' />
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</a>
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</td>
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</tr>
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<tr>
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<td>
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Gel of Plasmid PCR of promoters. Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors.
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</td>
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<td>
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Gel of BBa_K081005 digests from minipreps. Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid.
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</td>
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Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful.
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</td>
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</tr>
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</table>
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</html>
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==2011.06.26-2011.07.02==
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<html>
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<table class="gallery" frame='void' rules='none'>
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<tr>
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<td>
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<a name='20110628_TestDigest' href='https://2011.igem.org/File:20110628_TestDigest.jpg'>
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<img alt='20110628_TestDigest' src='https://static.igem.org/mediawiki/2011/5/51/20110628_TestDigest.jpg' />
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</a>
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Test to ensure all of our restriction enzymes are still active.  Lane 1: ladder; lane 2: cut with Eco RI; lane 3: cut with XbaI; lane 4: standard uncut DNA fragment for lanes 2 and 3; lane 5: cut with SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6.  There is an apparent decrease in size from standard DNA fragments to the digested samples.  In the digest lanes there is also a dim band fairly far down the gel corresponding to the small end piece that is the other product of digestion.
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</td>
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</tr>
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</table>
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</html>

Latest revision as of 17:03, 5 August 2011

Grinnell Menubar

Gels for Promoters

2011.06.12-2011.06.18

20110614_Promoters 20110616_promotorGel 20110616_promoterPostCleanupCrop
Figure 1: Promoters Pxyl (inducible) and PrsaA (constitutive). Lane 1: ladder; lane 2: PrsaA; Lane 3: Pxyl. Bands appear, but are hazy and spread out due to the small size of DNA fragments. Figure 2: PCR products of Caulobacter promoters PrsaA and Pxyl. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl. Figure 3: Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl.

2011.06.19-2011.06.25

20110620_promotorsTransformationGel 20110621 BBaGel1 20110622_PromotersGel2
Gel of Plasmid PCR of promoters. Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors. Gel of BBa_K081005 digests from minipreps. Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid. Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful.

2011.06.26-2011.07.02

20110628_TestDigest
Test to ensure all of our restriction enzymes are still active. Lane 1: ladder; lane 2: cut with Eco RI; lane 3: cut with XbaI; lane 4: standard uncut DNA fragment for lanes 2 and 3; lane 5: cut with SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6. There is an apparent decrease in size from standard DNA fragments to the digested samples. In the digest lanes there is also a dim band fairly far down the gel corresponding to the small end piece that is the other product of digestion.

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