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Sensor: Week 11 July 25-29
From 2011.igem.org
(Difference between revisions)
| Line 42: | Line 42: | ||
==Wednesday, July 27== | ==Wednesday, July 27== | ||
| + | {|border="2" | ||
| + | !Protocol | ||
| + | |align="center" colspan="2"|'''Part Involved with Protocol''' | ||
| + | |- | ||
| + | |Insert PCR | ||
| + | |colspan="2"|O<sub>R</sub> | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Insert using EcoRI and SpeI: | ||
| + | |O<sub>R</sub> | ||
| + | |- | ||
| + | |Vector using EcoRI and XbaI: | ||
| + | |34Cro | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|O<sub>R</sub>+34Cro | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2"|The above ligation was transformed into<br />Escherichia coli cells and plated onto<br />kanamycin resistant plates. | ||
| + | |} | ||
| + | The following constructs (assembled Tuesday, July 26) were verified on an agarose gel: | ||
| + | {| | ||
| + | |B0030 + C0051 + Or + K648000 | ||
| + | |- | ||
| + | |B0031 + C0051 + Or + K648000 | ||
| + | |- | ||
| + | |B0034 + C0051 + Or + K648000 | ||
| + | |} | ||
| + | ''Results:'' The gel indicated that the plasmids were the correct size. The colonies used for the gel were grown up in culture. | ||
| + | |||
| + | ==Thursday, July 28== | ||
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 19:40, 4 August 2011
Contents |
Monday, July 25
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Insert using EcoRI and SpeI: | B0034 |
| Vector using EcoRI and XbaI: | tev protease | |
| Ligation | B0034+tev protease | |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
OR+K64800 and OR were both grown up in culture to be used as freezer stocks.
Tuesday, July 26
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using EcoRI and SpeI: | B0031 + C0051 (R) B0030 + C0051 (R) B0034 + C0051 (R) |
| Vector using EcoRI and XbaI: | OR+K648000 | |
| Ligation | B0030 + C0051 + Or + K648000 B0031 + C0051 + Or + K648000 B0034 + C0051 + Or + K648000 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
The B0034+tev protease assembly from Monday, July 25 was verified on an agarose gel. The gel showed a band that corresponded to the correct size, so the colonies used for the gel were grown up in culture. The sequencing results came back positive.
Wednesday, July 27
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Insert PCR | OR | |
| Restriction Digest | Insert using EcoRI and SpeI: | OR |
| Vector using EcoRI and XbaI: | 34Cro | |
| Ligation | OR+34Cro | |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
The following constructs (assembled Tuesday, July 26) were verified on an agarose gel:
| B0030 + C0051 + Or + K648000 |
| B0031 + C0051 + Or + K648000 |
| B0034 + C0051 + Or + K648000 |
Results: The gel indicated that the plasmids were the correct size. The colonies used for the gel were grown up in culture.
