"
Sensor: Week 11 July 25-29
From 2011.igem.org
(Difference between revisions)
| Line 38: | Line 38: | ||
|colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin resistant plates. | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin resistant plates. | ||
|} | |} | ||
| + | |||
| + | The B0034+tev protease assembly from Monday, July 25 was verified on an agarose gel. The gel showed a band that corresponded to the correct size, so the colonies used for the gel were grown up in culture. The sequencing results came back positive. | ||
| + | |||
| + | ==Wednesday, July 27== | ||
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 19:35, 4 August 2011
Monday, July 25
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Insert using EcoRI and SpeI: | B0034 |
| Vector using EcoRI and XbaI: | tev protease | |
| Ligation | B0034+tev protease | |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
OR+K64800 and OR were both grown up in culture to be used as freezer stocks.
Tuesday, July 26
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using EcoRI and SpeI: | B0031 + C0051 (R) B0030 + C0051 (R) B0034 + C0051 (R) |
| Vector using EcoRI and XbaI: | OR+K648000 | |
| Ligation | B0030 + C0051 + Or + K648000 B0031 + C0051 + Or + K648000 B0034 + C0051 + Or + K648000 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
The B0034+tev protease assembly from Monday, July 25 was verified on an agarose gel. The gel showed a band that corresponded to the correct size, so the colonies used for the gel were grown up in culture. The sequencing results came back positive.
