Sensor: Week 11 July 25-29

From 2011.igem.org

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|colspan="2"|The above ligations weretransformed into Escherichia<br />coli cells and plated onto kanamycin<br />resistant plates.
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|colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin resistant plates.
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Revision as of 19:33, 4 August 2011

Monday, July 25

Protocol Part Involved with Protocol
Restriction Digest Insert using EcoRI and SpeI: B0034
Vector using EcoRI and XbaI: tev protease
Ligation B0034+tev protease
Transformation/Plating The above ligation was transformed into
Escherichia coli cells and plated onto
kanamycin resistant plates.

OR+K64800 and OR were both grown up in culture to be used as freezer stocks.

Tuesday, July 26

Protocol Part Involved with Protocol
Restriction Digest Inserts using EcoRI and SpeI: B0031 + C0051 (R)
B0030 + C0051 (R)
B0034 + C0051 (R)
Vector using EcoRI and XbaI: OR+K648000
Ligation B0030 + C0051 + Or + K648000
B0031 + C0051 + Or + K648000
B0034 + C0051 + Or + K648000
Transformation/Plating The above ligations were transformed into Escherichia
coli cells and plated onto kanamycin resistant plates.


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