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Sensor: Week 10 July 18-22
From 2011.igem.org
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Revision as of 17:22, 4 August 2011
Monday, July 18
The sensor group amplified the following constructs through the PCR protocol using the reverse primer:
| B0030+C0051 |
| B0031+C0051 |
| B0034+C0051 |
Tuesday, July 19
| Protocol | Part Involved in Protocol | |
|---|---|---|
| Restriction Digest | Inserts using EcoRI and SpeI: | B0030 + C0051 (R) B0031 + C0051 (R) B0034 + C0051 (R) Or B0034 |
| Vectors using EcoRI and XbaI: | Or34mcherry 34Cro Tev | |
| Ligation | B0030 + Or34mcherry B0031 + Or34mcherry B0034 + Or34mcherry Or + 34Cro B0034 + Tev | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
