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Sensor: Week 10 July 18-22
From 2011.igem.org
(Difference between revisions)
(Created page with "==Monday, July 18== The sensor group amplified the following constructs through the PCR protocol using the reverse primer: {| |B0030+C0051 |- |B0031+C0051 |- |B0034+C0051 |} ==...") |
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==Tuesday, July 19== | ==Tuesday, July 19== | ||
| + | {|border="1" | ||
| + | !Protocol | ||
| + | |colspan="2" align="center" |'''Part Involved in Protocol''' | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Inserts using EcoRI and SpeI: | ||
| + | |B0030 + C0051 (R)<br />B0031 + C0051 (R)<br />B0034 + C0051 (R)<br />Or<br />B0034 | ||
| + | |- | ||
| + | |Vectors using EcoRI and XbaI: | ||
| + | |Or34mcherry<br />34Cro<br />Tev | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|B0030 + Or34mcherry<br />B0031 + Or34mcherry<br />B0034 + Or34mcherry<br />Or + 34Cro<br />B0034 + Tev | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |The above ligations were transformed into<br />Escherichia coli cells and plated onto kanamycin resistant plates. | ||
| + | |} | ||
Revision as of 17:20, 4 August 2011
Monday, July 18
The sensor group amplified the following constructs through the PCR protocol using the reverse primer:
| B0030+C0051 |
| B0031+C0051 |
| B0034+C0051 |
Tuesday, July 19
| Protocol | Part Involved in Protocol | |
|---|---|---|
| Restriction Digest | Inserts using EcoRI and SpeI: | B0030 + C0051 (R) B0031 + C0051 (R) B0034 + C0051 (R) Or B0034 |
| Vectors using EcoRI and XbaI: | Or34mcherry 34Cro Tev | |
| Ligation | B0030 + Or34mcherry B0031 + Or34mcherry B0034 + Or34mcherry Or + 34Cro B0034 + Tev | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
