From 2011.igem.org
(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| - | <p style="display:block;"><font color="red">Edward</font>
| |
| | | | |
| - | </p><p style="display:block;"><u>Minipreps</u>
| |
| - | </p>
| |
| - | <ul><li>on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
| |
| - | </li><li>on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
| |
| - | </li><li>on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL
| |
| - |
| |
| - |
| |
| - | <p style="display:block;"><u>Restriction Digest of RBS-spoVg and T7 RNA polymerase </u>
| |
| - | <ul><li>BBa_K145001 cut by XbaI and PstI
| |
| - | </li><li>BBa_K143021 cut by SpeI and PstI
| |
| - |
| |
| - | <br />Final volume : 20 µL
| |
| - |
| |
| - | <u>DNA Gel electrophoresis (T7 RNA polymerase)</u>
| |
| - |
| |
| - | <ul><li>1kb ladder
| |
| - | </li><li>T7 undigested plasmid
| |
| - | </li><li>Digested T7 (BBa_K145001 cut by XbaI and PstI)
| |
| - |
| |
| - | </li></ul>
| |
| - | [[File:T7 electro 19072011.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]]
| |
| - | [[File:T7 electro 19072011cut.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]]
| |
Revision as of 14:25, 4 August 2011
"
