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Team:Paris Liliane Bettencourt/Notebook/2011/07/18/
From 2011.igem.org
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Revision as of 14:01, 4 August 2011
Edward
Minipreps
- on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
- on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
- on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL
Restriction Digest of RBS-spoVg and T7 RNA polymerase
- BBa_K145001 cut by XbaI and PstI
- BBa_K143021 cut by SpeI and PstI
Final volume : 20 µL</p><p style="display:block;">DNA Gel electrophoresis (T7 RNA polymerase) </p><p style="display:block;">Protocol (6 wells) </p>
- 1kb ladder
- T7 undigested plasmid
- Digested T7 (BBa_K145001 cut by XbaI and PstI)
ladder - non digested - digested T7
