Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

From 2011.igem.org

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</li><li>BBa_K143079 cut by  SpeI and PstI
</li><li>BBa_K143079 cut by  SpeI and PstI
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</p><p style="display:block;"><br />Final volume&nbsp;: 20 µL
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<br />Final volume&nbsp;: 20 µL

Revision as of 13:35, 4 August 2011

Edward

Minipreps

  • on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
  • on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
  • on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL


    Restriction Digest of RBS-spoVg and T7 RNA polymerase

    • BBa_K145001 cut by XbaI and PstI
    • BBa_K143079 cut by SpeI and PstI
      Final volume : 20 µL

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