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Copenhagen/2 August 2011
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* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector. | * Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector. | ||
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| + | *Image of gel: | ||
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| + | [[image:PCR gel 2-8-11.jpg|400px|right]] | ||
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* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result. | * Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result. | ||
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* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees. | * Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees. | ||
| - | * PCR on the A1 and B1 from the plates from yesterday - to see if its | + | * PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells and 3 colonies from transformed Bl21 cells. |
Latest revision as of 07:36, 3 August 2011
Tuesday
We are very excited about the gel we are going to make today!!
Labwork
- Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.
- Image of gel:
- Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
- Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
- PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells and 3 colonies from transformed Bl21 cells.
Other work
- Renew projectdescription
- Find a fungi induced promoter
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