Copenhagen/2 August 2011

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==Tuesday==
==Tuesday==
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We are very excited about the gel we are going to make today!!
'''Labwork'''
'''Labwork'''
* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.  
* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.  
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*Image of gel:
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[[image:PCR gel 2-8-11.jpg|400px|right]]
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* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
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* PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells  and 3 colonies from transformed Bl21 cells. 
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'''Other work'''
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* Renew projectdescription
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* Find a fungi induced promoter
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Back to [[Team:Copenhagen/Notebook|Notebook]]

Latest revision as of 07:36, 3 August 2011

Tuesday

We are very excited about the gel we are going to make today!!

Labwork

  • Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.
  • Image of gel:
PCR gel 2-8-11.jpg


  • Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
  • Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
  • PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells and 3 colonies from transformed Bl21 cells.


Other work

  • Renew projectdescription
  • Find a fungi induced promoter


Back to Notebook

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