Team:Paris Liliane Bettencourt/Notebook/2011/08/02/

(Difference between revisions)

Revision as of 10:34, 2 August 2011

First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics

Results of electroporation: IT WORKED after three trials!

Positive control worked
Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL or 5µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance.
Cm resistant strain have survived in low quantities at both 30 and 5 µg/mL of antibiotics
It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't anihilate all the bacteria.

Table of resutls of electroporation using Cao et al. method
tube 1: poration control no DNA (12,5 kV: ARC) CTL <power>+</power>: carpet CTL _- with Cm (5 and 30 µg/mL): none CTL _- with TetR (5 and 12,5 µg/mL): carpet	tube 2: poration control no DNA (10kV: OK) CTL ₊: carpet CTL _- with Cm: none CTL _- with TetR: none	tube 3: pHM3::TetR (12,5 kV: OK) TetR(5 and 12,5 µg/mL): carpet	tube 4: pHM3::TetR (10 kV: OK) TetR (12,5 µg/mL): carpet	tube5: pHM3::TetR (12,5 kV: ARC) TetR (5 and 12,5 µg/mL): carpet	tube 6: pHM3::TetR (10 kV: OK) TetR (5 µg/mL): carpet
tube 7: S12::Cm (12,5 kV: ARC) Cm 5 µg/mL: 1 colony Cm 30 µg/mL: 1 colony	tube 8: S12::Cm (10 kV: OK) Cm 30 µg/mL: 4 colonies	tube 9: S12::Cm (12,5 kV: OK) Cm 30 µg/mL: 9 colonies Cm 5 µg/mL: 4 colonies	tube 10: S12::Cm (10 kV: OK) Cm 5 µg/mL: 1 colonie	CTL _--: no bacteria no growth

@@ Line 19: / Line 19: @@
 |-
 |tube 1: poration control no DNA (12,5 kV: ARC)
-*CTL <sub>+</sub>: carpet
+*CTL <power>+</power>: carpet
 *CTL <sub>-</sub> with Cm (5 and 30 µg/mL): none
 *CTL <sub>-</sub> with TetR (5 and 12,5 µg/mL): carpet