Team:Caltech/Notebook

From 2011.igem.org

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<p>[[Team:Caltech/Week_5|Week 5]]: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit</p>
<p>[[Team:Caltech/Week_5|Week 5]]: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit</p>
<p>[[Team:Caltech/Week_6|Week 6]]: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit</p>
<p>[[Team:Caltech/Week_6|Week 6]]: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit</p>
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<p>[[Team:Caltech/Week_7|Week 7]]: 7/24-7/30</p>: p450 testing, plating of EDC's, more assembly
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<p>[[Team:Caltech/Week_7|Week 7]]: 7/24-7/30: p450 testing, plating of EDC's, more assembly</p>
<p>[[Team:Caltech/Week_8|Week 8]]: 7/31-8/6</p>
<p>[[Team:Caltech/Week_8|Week 8]]: 7/31-8/6</p>
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Revision as of 23:45, 1 August 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

Notebook

Methods and Protocols

Week 1: 6/15-6/17: Lab orientation

Week 2: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples

Week 3: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures

Week 4: 7/3-7/10: Sequencing and testing of Gibson assembled vectors

Week 5: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit

Week 6: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit

Week 7: 7/24-7/30: p450 testing, plating of EDC's, more assembly

Week 8: 7/31-8/6


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