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	[[Media: T4-ligase.pdf | Using T4 ligase]]		[[Media: T4-ligase.pdf | Using T4 ligase]]
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		+	[[Media: digests.pdf | Using Restriction enzymes]]

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Revision as of 16:21, 1 August 2011

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Protocols

Basic protocols ::: These protocols are necessary for all lab operations.

Autoclave usage

OD600 optimization

Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.

Gel electrophoresis

Using the gel viewer

Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Production of electrocompetent cells

Electroporation of electrocompetent cells

Production of standard/QT competent cells

Quick Transformation (QT) of standard competent cells

Heat shock transformation of standard competent cells.

Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.

Using quick ligase

Using T4 ligase

Using Restriction enzymes

Biobrick specific ::: These protocols contain information specific to the usage of Biobricks.

Extraction of BioBricks from wells in the distribution