Team:Freiburg/Notebook/1 August

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Contents 1 green light receptor 1.1 NAME OF YOUR EXPERIMENT 2 blue light receptor 2.1 NAME OF YOUR EXPERIMENT 3 red light receptor 3.1 NAME OF YOUR EXPERIMENT 4 Lysis cassette 4.1 NAME OF YOUR EXPERIMENT 5 Precipitator

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME: Sophie PCR

Name: Sophie	Date: 01.08.11
Continue from Experiment: PCR (Date): 28.07.11 (Name): Sandra, Sophie
Project Name: Blue Light

PCR-Mixture for one Reaction:

For a 50 µl reaction use

32,5µl	H20	Name
10µl	5x Phusion Buffer	of Primer
2.5µl	Primer fw	P35
2.5µl	Primer dw	P36
1µl	dNTPs	of Template DNA
1µl	DNA-Template	M35 (LovTAP)
0.5 µl	Phusion (add in the end)

What program do you use?

LovTAP ohne Ueberhaenge

To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.

How did you label the PCR-Product, where is it stored and what do you do next?

Labelled: M35 short; stored in Gibson Stuff box.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

PCR

Name: Sophie	Date: 01.08.11
Continue from Experiment: replay of PCR 19.07.11 (Date) 19.07.11 (Name) Ruediger
Project Name: GFP Pbd

PCR-Mixture for one Reaction:

For a 50 µl reaction use

32,5µl	H20	Name
10µl	5x Phusion Buffer	of Primer
2.5µl	Primer fw	P28
2.5µl	Primer dw	P18 / P19/ P20
1µl	dNTPs	of Template DNA
1µl	DNA-Template	S 14 (GFP)
0.5 µl	Phusion (add in the end)

What program do you use?

Ruediger PCR (modified by Tobi and me. First 10 cycles with 55°C, next cycles with 62°C annealing temperature.

To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.

How did you label the PCR-Product, where is it stored and what do you do next?