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Team:British Columbia/Notebook/4 July 2011
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==July 04 2011== | ==July 04 2011== | ||
| - | + | <b>IDI1, HMG2, erg20-2</b> | |
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*Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | *Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | ||
*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | *Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | ||
*Awaiting materials from France (erg20-2). | *Awaiting materials from France (erg20-2). | ||
| - | + | <p><b>1,8-Cineole</b></p> | |
| - | + | *Ran a gel to test restriction digest on variant SDM-PCR product. Looks like the SDM was successful! | |
| - | *Ran a gel to test restriction digest | + | <p><b>beta-pinene & (-)-limonene</b></p> |
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*SDM beta-pinene again by using both Pfu and Taq. | *SDM beta-pinene again by using both Pfu and Taq. | ||
*Gel verification shows a smear. | *Gel verification shows a smear. | ||
*Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested. | *Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested. | ||
Latest revision as of 20:04, 30 July 2011
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July 04 2011
IDI1, HMG2, erg20-2
- Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
- Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
- Awaiting materials from France (erg20-2).
1,8-Cineole
- Ran a gel to test restriction digest on variant SDM-PCR product. Looks like the SDM was successful!
beta-pinene & (-)-limonene
- SDM beta-pinene again by using both Pfu and Taq.
- Gel verification shows a smear.
- Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested.
