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Team:Freiburg/Notebook/29 July
From 2011.igem.org
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==Meeting== | ==Meeting== | ||
| - | attendants: | + | attendants: Julia, Manuel, Rüdiger, Sandra, Theo, Tobi |
| + | Time: 9:00 - 11:00 | ||
===<span style="color:green;">green light receptor</span>=== | ===<span style="color:green;">green light receptor</span>=== | ||
already done: | already done: | ||
| - | + | *Ccas has to sites where we need to do a mutagenesis (quick change), both were done seperately, but the no colonies showed up (wrong PCR program) | |
| + | *CcaR has one site where we need to do a mutagenesis (quick change), this has been done, colonies were growing | ||
To-do: | To-do: | ||
| - | + | *miniprep, digestion and sequencing of the CcaR clones | |
| + | *do the Ccas PCR again | ||
| + | *next steps would be the big assembly wiht pcyA | ||
===<span style="color:blue;">blue light receptor</span>=== | ===<span style="color:blue;">blue light receptor</span>=== | ||
| - | already done: PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work. | + | already done: |
| + | *PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work. | ||
Revision as of 09:39, 29 July 2011
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