Team:Edinburgh/Project

From 2011.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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{{:Team:Edinburgh/tech/Navbox}}
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<html><script type="text/javascript" >$(document).ready(function() {
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getMenus('home', 'home_project');
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<div class="main_body">
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__NOTOC__
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'''This page is obsolete. The project should be documented at the relevant subpages:'''
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* [[Team:Edinburgh/Phage Reactors 2.0|Phage Reactors]]
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* [[Team:Edinburgh/Cell Display|Cell Display]]
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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* [[Team:Edinburgh/Biorefineries|Biorefineries]]
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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==Designs==
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{|align="justify"
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The general formats for the basic phage and cell display would be:
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Edinburgh_logo.png|200px|right|frame]]
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|-
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Edinburgh_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:Edinburgh | Team Example]]
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|}
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<!--- The Mission, Experiments --->
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* Promoter-RBS-LeaderSequence-Enzyme-(Linker?)-pVIII
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* Promoter-RBS-INP-(Linker?)-Enzyme
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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==Actions that ought to be carried out==
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!align="center"|[[Team:Edinburgh|Home]]
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!align="center"|[[Team:Edinburgh/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Edinburgh Official Team Profile]
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!align="center"|[[Team:Edinburgh/Project|Project]]
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!align="center"|[[Team:Edinburgh/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Edinburgh/Modeling|Modeling]]
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!align="center"|[[Team:Edinburgh/Notebook|Notebook]]
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!align="center"|[[Team:Edinburgh/Safety|Safety]]
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!align="center"|[[Team:Edinburgh/Attributions|Attributions]]
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|}
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===General===
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* Make or acquire fusion-ready cellulases (e.g. <partinfo>BBa_K392006</partinfo>, <partinfo>BBa_K392007</partinfo>, <partinfo>BBa_K392008</partinfo>).
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* Since a simple test system is desired, make fusion-ready amylase, from ''E. coli's'' own ''malS'' gene.
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== '''Overall project''' ==
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* Make or acquire spacer/linker sequences?
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** Short linkers could be ordered as oligos. [[Team:Edinburgh/Berkeley 2009 Parts|Berkeley 2009 made some longer linkers]] which we could order.
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Your abstract
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===Phage display===
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* Acquire M13 phage.
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* PCR from our [http://www.neb.com/nebecomm/products/productn4018.asp M13mp18] to get:
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** pVIII mature protein
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** pVIII leader sequence, coding for: MKKSLVLKASVAVATLVPMLSFA.
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* Make or acquire fusion-ready pIII gene? (optional)
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** See <partinfo>BBa_K415138</partinfo> (complete) or <partinfo>BBa_K257001</partinfo> (''sans'' leader sequence).
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** Our final fusion will need the leader sequence followed by the gene of interest followed by pIII proper.
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===Cell display===
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* Make or acquire fusion-ready cell-surface display parts.
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** See [https://2009.igem.org/Team:Berkeley_Wetlab/Cell_Surface_Display_Parts Berkeley 2009] (but perhaps ignore them).
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** Instead, <partinfo>BBa_K265008</partinfo> (ice nucleation protein) looks promising.
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** The ''fliC'' gene seems to be the target for flagella display; note that the displayed protein has to be inserted into the middle of it.
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===Biorefinery===
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== Project Details==
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:''To add...''
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===Completion===
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* Fuse whatever enzymes to cell-display parts or pVIII or maybe pIII.
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* ???
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* '''Profit!'''
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=== Part 2 ===
 
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 14:41, 28 July 2011

This page is obsolete. The project should be documented at the relevant subpages:

Designs

The general formats for the basic phage and cell display would be:

  • Promoter-RBS-LeaderSequence-Enzyme-(Linker?)-pVIII
  • Promoter-RBS-INP-(Linker?)-Enzyme

Actions that ought to be carried out

General

  • Make or acquire fusion-ready cellulases (e.g. <partinfo>BBa_K392006</partinfo>, <partinfo>BBa_K392007</partinfo>, <partinfo>BBa_K392008</partinfo>).
  • Since a simple test system is desired, make fusion-ready amylase, from E. coli's own malS gene.

Phage display

  • Acquire M13 phage.
  • PCR from our [http://www.neb.com/nebecomm/products/productn4018.asp M13mp18] to get:
    • pVIII mature protein
    • pVIII leader sequence, coding for: MKKSLVLKASVAVATLVPMLSFA.
  • Make or acquire fusion-ready pIII gene? (optional)
    • See <partinfo>BBa_K415138</partinfo> (complete) or <partinfo>BBa_K257001</partinfo> (sans leader sequence).
    • Our final fusion will need the leader sequence followed by the gene of interest followed by pIII proper.

Cell display

  • Make or acquire fusion-ready cell-surface display parts.
    • See Berkeley 2009 (but perhaps ignore them).
    • Instead, <partinfo>BBa_K265008</partinfo> (ice nucleation protein) looks promising.
    • The fliC gene seems to be the target for flagella display; note that the displayed protein has to be inserted into the middle of it.

Biorefinery

To add...

Completion

  • Fuse whatever enzymes to cell-display parts or pVIII or maybe pIII.
  •  ???
  • Profit!


Retrieved from "http://2011.igem.org/Team:Edinburgh/Project"