Team:Freiburg/Notebook/28 July

From 2011.igem.org

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*0.5 μl PStI<br/>
*0.5 μl PStI<br/>
*3 μl DNA (M45a-M45d)<br/>
*3 μl DNA (M45a-M45d)<br/>
 +
 +
Incbation for 1 hour. Gel was loaded afterwards.
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 10:07, 28 July 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Digestion of PCR product

Investigators:Sandra

Digest of PCR product S35 (Lov-tap), because PCR did not work out. We got a band of 1000bp, but we expected 3000bp.
Digest:

  • 1 μl NEB4 buffer
  • 1 μl BSA
  • 1 μl NcoI
  • 2 μl H2O
  • 5 μl S35

Incubation for 30 min 37°C.
Afterwards the digested product was loaded onto gel.

Miniprep of Not-Gate

Investigators: Sophie

Picked colonies from plate and then performed a miniprep.

DNA-concentration measured with nanodrop:

sample DNA-concentration (ng/μl)
M45a 248.9
M45b 256.8
M45c 291.3
M45d 295.8


Testdigest of Miniprep

Investigators: Sophie

Testdigest of M45a-M45d.

  • 4 μl H20
  • 1 μl NEB4
  • 1 μl BSA
  • 0.5 μl EcoRI
  • 0.5 μl PStI
  • 3 μl DNA (M45a-M45d)

Incbation for 1 hour. Gel was loaded afterwards.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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