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Team:Freiburg/Notebook/28 July
From 2011.igem.org
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==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
| - | === | + | ===Digestion of PCR product=== |
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| - | + | ||
| + | '''Investigators:Sandra''' | ||
| + | Digest of PCR product S35 (Lov-tap), because PCR did not work out. We got a band of 1000bp, but we expected 3000bp. | ||
| + | <br/> | ||
| + | Digest:<br/> | ||
| + | *1 μl NEB4 buffer<br/> | ||
| + | *1 μl BSA<br/> | ||
| + | *1 μl NcoI<br/> | ||
| + | *2 μl H2O<br/> | ||
| + | *5 μl S35<br/> | ||
| + | Incubation for 30 min 37°C.<br/> | ||
| + | Then all of it--> gel.<br/> | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Revision as of 09:42, 28 July 2011
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