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Team:UST-Beijing/Project
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We are engineering a bile acid sensor in E.coli and others | We are engineering a bile acid sensor in E.coli and others | ||
| + | Using lacI DNA-binding domain and LXRbeta ligand-binding domain, we made an artificial bile acid receptor which can regulate expression of target gene within a natural lacI operon. As proof of principle, we demonstrate that regulation of bacteria gene expression by host eukaryocyte metabolites is achievable using chimeric nuclear receptors. Through directed molecular evolution, a harmonious signal network regulating metabolism of both prokaryocytes and their host eukaryocytes in the digestive tract is feasible. | ||
== Project Details== | == Project Details== | ||
Revision as of 04:53, 27 July 2011
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Overall project
We are engineering a bile acid sensor in E.coli and others
Using lacI DNA-binding domain and LXRbeta ligand-binding domain, we made an artificial bile acid receptor which can regulate expression of target gene within a natural lacI operon. As proof of principle, we demonstrate that regulation of bacteria gene expression by host eukaryocyte metabolites is achievable using chimeric nuclear receptors. Through directed molecular evolution, a harmonious signal network regulating metabolism of both prokaryocytes and their host eukaryocytes in the digestive tract is feasible.
