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Revision as of 17:49, 17 July 2011 (view source) Pon (Talk | contribs) ← Older edit		Latest revision as of 21:23, 21 July 2011 (view source) TravisCR (Talk | contribs) (→Timeline)
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-	==Transformation of Dpn1-digested plasmid==	+	=Transformation of Dpn1-digested plasmid=
	17 July 2011 1pm		17 July 2011 1pm
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	* LB media instead of SOC.		* LB media instead of SOC.
-	Transformanda on ice at 1305h.	+	==Timeline==
-	Heat shocked @ 1335h, incubating at 37˚C.	+	* Transformanda on ice at 1305h.
		+	* Heat shocked @ 1335h.
		+	* incubating at 37˚C until 1415.
		+
		+
		+	[[Team:Baltimore/Notebook]]

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Latest revision as of 21:23, 21 July 2011

Transformation of Dpn1-digested plasmid

17 July 2011 1pm

Transformed contents of tubes 3a, 3b, 4a using transformation protocol with the following changes:

• 2µl of mutagenesis mix used instead of 1µl.
• LB media instead of SOC.

Timeline

• Transformanda on ice at 1305h.
• Heat shocked @ 1335h.
• incubating at 37˚C until 1415.

Team:Baltimore/Notebook