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Team:Lyon-INSA-ENS/Realisation/Week5
From 2011.igem.org
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<h6 style="text-align :left"> Monday </h6> | <h6 style="text-align :left"> Monday </h6> | ||
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Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells. | Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells. | ||
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<h6 style="text-align :left"> Tuesday </h6> | <h6 style="text-align :left"> Tuesday </h6> | ||
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Transformation of the ligations into NM522 using a CaCl2 chemical transformation. | Transformation of the ligations into NM522 using a CaCl2 chemical transformation. | ||
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<h6 style="text-align :left"> Wednesday </h6> | <h6 style="text-align :left"> Wednesday </h6> | ||
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<p style = "line-height : 1.5em"> | <p style = "line-height : 1.5em"> | ||
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<h6 style="text-align :left"> Thursday </h6> | <h6 style="text-align :left"> Thursday </h6> | ||
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Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed. | Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed. | ||
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Revision as of 09:27, 21 July 2011
Week 5
From Monday the 4th of July to Friday the 8th of July 2011
Monday
Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.
Tuesday
Transformation of the ligations into NM522 using a CaCl2 chemical transformation.
Wednesday
Selection of individual transformed colonies and start of solid and liquid culture.
We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.
Thursday
Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed.
