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Team:Cornell/Week 7
From 2011.igem.org
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==Wednesday== | ==Wednesday== | ||
Lab Work Done By: James Mathew | Lab Work Done By: James Mathew | ||
| - | + | *Set up ligation reaction of pZE-12 backbone with the primer dimer insert. | |
| - | Set up ligation reaction of pZE-12 backbone with the primer dimer insert. | + | |
Digestion of Backbone | Digestion of Backbone | ||
- 26 ul Backbone plasmid = 2 ug DNA | - 26 ul Backbone plasmid = 2 ug DNA | ||
| Line 49: | Line 48: | ||
Label: | Label: | ||
| - | + | *Ligation done overnight for transformation on 7/21/11 | |
==Thursday== | ==Thursday== | ||
Revision as of 20:01, 20 July 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
July 17th - July 23rd
Sunday
Lab work done by: Alyssa Henning & Bill Jo
- Successfully miniprepped 1 sample of GFP + avitag (the second sample got messed up)
- Ran a gel on the RFP that was PCRed on Saturday. We also cut out the bands.
- Sean will innoculate 6 tubes of GFP-containing bacteria--1 tube for us, and 5 tubes for CURIE.
Monday
Lab work done by: Charlie Chung & Youjin Cho
- Gel purified 2 RFP PCR samples from Friday.
- Miniprepped 2 samples of GFP + avitag.
Tuesday
Lab Work Done By: James Mathew
- Submitted GFP+avitag genes for synthesis
- Submitted pZE-12 backbone for subcloning of light sensor
Wednesday
Lab Work Done By: James Mathew
- Set up ligation reaction of pZE-12 backbone with the primer dimer insert.
Digestion of Backbone
- 26 ul Backbone plasmid = 2 ug DNA
- 5 ul Buffer #4 (optimal for SphI-HF and ClaI-HF)
- 1 ul SphI & 1 ul ClaI
- 16.5 ul H20
left in water bath for one hour
- 1 ul CIAP added to digestion reaction
left in water bath for 30 minutes
Ran Digestion Product through gel for 30 minutes at 120 V.
Lane 1: 2 ul 1kb Ladder + 2 ul 6X Dye + 8 ul H20
Lane 2: 25 ul digestion reaction + 5 ul 6X Dye
Lane 3: 25 ul digestion reaction + 5 ul 6X Dye
Gel Purification using Qiagen Kit
NanoDrop of Samples
Concentration:
Label:
Ligation Reaction
Label:
- Ligation done overnight for transformation on 7/21/11
