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Team:Caltech/Week 6
From 2011.igem.org
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==July 18== | ==July 18== | ||
| - | + | Colony PCR of positive Gibson plates from Friday<br/> | |
| - | + | Gel of short PCR parts to confirm they are being amplified<br/> | |
| - | + | Pulsed field gel with a larger amount of soil sample DNA<br/> | |
| - | Retransform pSB3K5 into Xl-10s<br> | + | Retransform pSB3K5 into Xl-10s<br/> |
===Results=== | ===Results=== | ||
==July 19== | ==July 19== | ||
PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control<br> | PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control<br> | ||
===Results=== | ===Results=== | ||
| - | pSB3K5 plate had 0 colonies.<br> | + | pSB3K5 plate had 0 colonies.<br/> |
| + | PFGE of soil-extracted DNA shows a smear; will do gel extraction of ~40kbp sequences for fosmid kit. | ||
Gibson PCR concentrations | Gibson PCR concentrations | ||
Revision as of 18:59, 20 July 2011
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Project |
July 18Colony PCR of positive Gibson plates from Friday ResultsJuly 19PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control ResultspSB3K5 plate had 0 colonies. Gibson PCR concentrations
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