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From 2011.igem.org

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-	==Notebook==	+	==Calender/Notebook==
-	You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.	+	'''April 13th:'''
		+
		+	Officially met for the first time and discussed ideas for our project. Some of the original ideas included a strain of yeast that acted as a water tester, a strain of yeast that emitted a cinnomon scent, and a strain of yeast that reverses the oxydation of copper.
		+
		+	'''April 20th:'''
		+
		+	After we read an article on hw yeast copes with toxic metals (Wysocki & Tamas, 2010), we made the final decision of making a water tester. We discussed what metals we wanted to detect in an environment, and we decided on copper, becase yeast already has a promoter associated with copper, Cup1.
		+
		+	'''April 23th:'''
		+
		+	We met early in the morning and discussed how to actually make our plasmid. We read about and decided to use Ura3 as our building block, and that we were going to put our plasmid in Ura3 (Mumberg, Muller, & Funk, 1995). We learned about the Gibson Method, a new system of putting together DNA parts instead of using restrictive enzymes. We also learned the order of our part: Promoter, Kozak Sequence, Space, Protein Coding Region, and then Terminator.
		+
		+	'''April 37th:'''

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Revision as of 20:45, 9 May 2011

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.	[[Image:|200px|right|frame]]
Our project this year is to design, create, and test a working cadmium water tester. We are going to do this by altering the existing biochemical pathway that Yeast has to recognize the cadmium, and then create our own code that will make it fluoresce red. When we originally had the idea for a water tester, we looked for metals, pesticides, and other harmful substances that are a problem in water supplies. Through much research, we found that cadmium is often a byproduct of many industrial processes, and is often leaked or exposed to the environment. Also, yeast recognizes the presence of cadmium already, so we figured it would be much more simple to alter an existing pathway than to create an entirely new one. We hope through research and experimentation that we are able to make cadmium much more detectable in the environment.
	Greenfield-Central High School Team Rihm

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Calender/Notebook

April 13th:

Officially met for the first time and discussed ideas for our project. Some of the original ideas included a strain of yeast that acted as a water tester, a strain of yeast that emitted a cinnomon scent, and a strain of yeast that reverses the oxydation of copper.

April 20th:

After we read an article on hw yeast copes with toxic metals (Wysocki & Tamas, 2010), we made the final decision of making a water tester. We discussed what metals we wanted to detect in an environment, and we decided on copper, becase yeast already has a promoter associated with copper, Cup1.

April 23th:

We met early in the morning and discussed how to actually make our plasmid. We read about and decided to use Ura3 as our building block, and that we were going to put our plasmid in Ura3 (Mumberg, Muller, & Funk, 1995). We learned about the Gibson Method, a new system of putting together DNA parts instead of using restrictive enzymes. We also learned the order of our part: Promoter, Kozak Sequence, Space, Protein Coding Region, and then Terminator.

April 37th: