Team:Caltech/Week 6

From 2011.igem.org

(Difference between revisions)
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===Results===
===Results===
==July 19==
==July 19==
 +
PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control<br>
===Results===
===Results===
pSB3K5 plate had 0 colonies.<br>
pSB3K5 plate had 0 colonies.<br>
 +
 +
Gibson PCR concentrations
 +
<table border="1">
 +
<tr>
 +
<th>Part</th>
 +
<th>Concentration (ng/ul)</th>
 +
</tr>
 +
<tr>
 +
<td>R0010 for pNT001</td>
 +
<td>86.4</td>
 +
</tr>
 +
<tr>
 +
<td>B0014 for pNT001</td>
 +
<td>128.3</td>
 +
</tr>
 +
<tr>
 +
<td>B0014 for pNT003</td>
 +
<td>89.1</td>
 +
</tr>
 +
<tr>
 +
<td>promoter for GFP Gibson positive control</td>
 +
<td>51.9</td>
 +
</tr>
 +
<tr>
 +
<td>GFP for positive control</td>
 +
<td>130.4</td>
 +
</tr>
 +
<tr>
 +
<td>terminator for positive control</td>
 +
<td>82.5</td>
 +
</tr>
 +
</table>
}}
}}

Revision as of 18:54, 20 July 2011


Caltech iGEM 2011



Home

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References

Support

July 18

Run colony PCR of positive Gibson plates from Friday
Run gel of short PCR parts to confirm they are being amplified
Run pulsed field gel with a larger amount of soil sample DNA
Retransform pSB3K5 into Xl-10s

Results

July 19

PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control

Results

pSB3K5 plate had 0 colonies.

Gibson PCR concentrations

Part Concentration (ng/ul)
R0010 for pNT001 86.4
B0014 for pNT001 128.3
B0014 for pNT003 89.1
promoter for GFP Gibson positive control 51.9
GFP for positive control 130.4
terminator for positive control 82.5


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