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Uppsala-Sweden/27 June 2011
From 2011.igem.org
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| + | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2011 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2011 | month=07}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2011 | month=08}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2011 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2011 | month=10}} | ||
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| + | <div class="prefix_4 grid_11 suffix_1 maincontent" id="Uppsalacontent"> | ||
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| + | 2011-06-27 | ||
This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 10^8 transformants /ug DNA. | This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 10^8 transformants /ug DNA. | ||
Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA. | Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA. | ||
Latest revision as of 16:28, 20 July 2011
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-06-27
This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 10^8 transformants /ug DNA.
Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA.
