From 2011.igem.org
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| - | <td>18</td>
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| - | <td>2.5</td>
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| - | <td>1 XbaI</td>
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| - | <td>1 PstI</td>
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| - | <td>2.5</td>
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| - | <td>25</td>
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Revision as of 21:35, 19 July 2011
UNIPV TEAM 2011
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July, 11th
| Plasmid |
Kind |
DNA (μl) |
H2O (μl) |
Enzyme 1 (μl) |
Enzyme 2 (μl) |
Buffer H (μl) |
Final Volume (μl) |
| E1-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E2-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E3-1 |
|
18 |
2.5 |
1 EcoRI |
1 SpeI |
2.5 |
25 |
| E4-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E5-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E6-1 |
|
11 |
9.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E7-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E8-3 |
|
20.5 |
0 |
1 SpeI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_R0040</partinfo> |
|
14.5 |
6 |
1 SpeI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_C0261</partinfo> |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_I13507</partinfo> |
|
10 |
10.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_B0034</partinfo> |
|
10 |
10.5 |
1 SpeI |
1 PstI |
2.5 |
25 |
|
"
