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Team:WashU/Notebook/May
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| - | == May == | + | ===May 31=== |
| + | |||
| + | *Preliminary Shopping List | ||
| + | |||
| + | ##PCR buffer w/o MgCl - 5ML - $45.00 | ||
| + | ##M8787- 5ML -MgCl Reagent- $49.30 | ||
| + | ##D7295-.5ML - dNTP - $73.00 | ||
| + | ##D4184-250UN - Taq Polymerase - $150.00 | ||
| + | ##D8045-250UN - AccuTaq - $300 | ||
| + | ##E1385-5ML – Ethidium Bromide – $23.70 | ||
| + | ##T8280-1L – Tris Acetate EDTA Buffer – $45.10 | ||
| + | ##NA1020-1KT – PCR Clean-Up Kit -$102.50 | ||
| + | ##NA1111-1KT - Gel Extraction Kit - $102.00 | ||
| + | |||
| + | Subtotal: $890.60 | ||
| + | |||
| + | *Met with Cohen Lab grad students to finalize plan and get cassettes. | ||
| + | *Received Leu2 cassette in pRS305 plasmid. | ||
| + | *Received Ura3 cassette in pRS306 plasmid. | ||
| + | |||
| + | ===June 1=== | ||
| + | |||
| + | *Lesson from Bert Berla on how to design primers | ||
| + | *Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm | ||
| + | **Double checked gene and restriction sites to make sure enzyme does not cut in the middle of the gene | ||
| + | *Design Primers for cassettes | ||
| + | **Ura3, Leu2, KanMX4, and NatMX4 | ||
Revision as of 02:04, 19 July 2011
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May 31
- Preliminary Shopping List
- PCR buffer w/o MgCl - 5ML - $45.00
- M8787- 5ML -MgCl Reagent- $49.30
- D7295-.5ML - dNTP - $73.00
- D4184-250UN - Taq Polymerase - $150.00
- D8045-250UN - AccuTaq - $300
- E1385-5ML – Ethidium Bromide – $23.70
- T8280-1L – Tris Acetate EDTA Buffer – $45.10
- NA1020-1KT – PCR Clean-Up Kit -$102.50
- NA1111-1KT - Gel Extraction Kit - $102.00
Subtotal: $890.60
- Met with Cohen Lab grad students to finalize plan and get cassettes.
- Received Leu2 cassette in pRS305 plasmid.
- Received Ura3 cassette in pRS306 plasmid.
June 1
- Lesson from Bert Berla on how to design primers
- Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
- Double checked gene and restriction sites to make sure enzyme does not cut in the middle of the gene
- Design Primers for cassettes
- Ura3, Leu2, KanMX4, and NatMX4
