Team:Baltimore/Notebook/Transformation
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Revision as of 17:49, 17 July 2011
Transformation of Dpn1-digested plasmid
17 July 2011 1pm
Transformed contents of tubes 3a, 3b, 4a using transformation protocol with the following changes:
- 2µl of mutagenesis mix used instead of 1µl.
- LB media instead of SOC.
Transformanda on ice at 1305h. Heat shocked @ 1335h, incubating at 37˚C.