Team:UNIPV-Pavia/Calendar/July/settimana2

From 2011.igem.org

(Difference between revisions)
Line 122: Line 122:
       <td>17.5</td>
       <td>17.5</td>
       <td>1 EcoRI</td>
       <td>1 EcoRI</td>
 +
      <td>1 PstI</td>
 +
      <td>2.5</td>
 +
      <td>25</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td></html><partinfo>BBa_I13501</partinfo><html></td>
 +
      <td>Insert</td>
 +
      <td>7.2</td>
 +
      <td>13.3</td>
 +
      <td>1 XbaI</td>
       <td>1 PstI</td>
       <td>1 PstI</td>
       <td>2.5</td>
       <td>2.5</td>
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</center>
</center>
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+
<p>
 +
A small-size and a medium-size agarose gel were prepared according to protocols.
 +
</p>
<div align="right"><small><a href="#indice" title="">^top</a></small></div>
<div align="right"><small><a href="#indice" title="">^top</a></small></div>

Revision as of 14:54, 16 July 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

JULY: WEEK 2

July, 4th

Digestion of previously purified plasmids were performed for ligations:

Plasmid Kind Purified Dna (μl) H2O (&mul) Enzyme 1 (μl) Enzyme 2 (μl) Buffer H (μl) Final Volume(μl)
<partinfo>BBa_C0060</partinfo> Insert 16.5 4 1 EcoRI 1 SpeI 2.5 25
<partinfo>BBa_C0061</partinfo> Insert 13.2 7.3 1 XbaI 1 PstI 2.5 25
<partinfo>BBa_K081022</partinfo> Insert 15.7 4.8 1 EcoRI 1 PstI 2.5 25
<partinfo>BBa_B0030</partinfo> Vector 13.2 7.3 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0031</partinfo> Vector 12.4 8.1 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0032</partinfo> Vector 9.5 11 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0015</partinfo> Vector 7.9 12.6 1 EcoRI 1 XbaI 2.5 25
<partinfo>pSB4C5</partinfo> Vector 3 17.5 1 EcoRI 1 PstI 2.5 25
<partinfo>BBa_I13501</partinfo> Insert 7.2 13.3 1 XbaI 1 PstI 2.5 25

A small-size and a medium-size agarose gel were prepared according to protocols.