Team:Freiburg/Notebook/13 July

From 2011.igem.org

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|-
|-
|M2a beta/M2a* beta
|M2a beta/M2a* beta
 +
|83.8/13.8
 +
|1.80/1.72
 +
|1.52/0.97
 +
|-
 +
|M2a gamma/M2a* gamma
 +
|118.9/56.1
 +
|1.68/1.86
 +
|1.00/2.13
 +
|-
 +
|M2a delta/M2a* delta
 +
|101.5/66.0
 +
|1.74/1.85
 +
|1.26/2.16
 +
|-
 +
|M2a epsilon/M2a* epsilon
 +
|112.9/69.9
 +
|1.68/1.82
 +
|1.00/1.82
 +
|-
 +
|M2b alpha/M2a* alpha
 +
|79.7/74.9
 +
|1.87/1.83
 +
|2.20/2.14
 +
|-
 +
|M2b Beta/M2b* beta
 +
|122.3/74.4
 +
|1.71/1.84
 +
|1.04/2.18
 +
|-
 +
|M2b gamma/M2b* gamma
 +
|111.2/72.0
 +
|1.70/1.83
 +
|1.08/1.99
 +
|-
 +
|M2b epsilon/M2b* epsilon
 +
|88.5/90.09
 +
|1.72/1.79
 +
|1.19/1.78
 +
|-
 +
|M3 alpha/M3* alpha
 +
|136.3/93.9
 +
|1.71/1.78
 +
|1.10/1.74
 +
|-
 +
|M3 beta/M3* beta
 +
|54.7/79.2
 +
|1.91/1.83
 +
|2.03/2.13
 +
|-
 +
|M3 gamma/M3* gamma
 +
|130.6/141.7
 +
|1.66/1.80
 +
|0.87/1.88
 +
|-
 +
|M3 delta/M3* delta
 +
|79.6/82.9
 +
|1.76/1.85
 +
|1.27/2.19
 +
|-
 +
|M3 epsilon/M3* epsilon
 +
|84.1/122.5
 +
|1.79/1.69
 +
|1.45/1.03
|}
|}

Revision as of 12:43, 15 July 2011

Contents

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples DNA concentration 260/280 230
cph8a 33.7 1.79 1.74
cph8b 41.2 1.79 1.39
cph8c 24.1 1.87 1.47
cph8d 30 1.74 1.84
cph8e 34 1.81 1.86
cph8f 30 1.83 1.81


Testdigest

Investigators: Julia

Testdigest of cph8

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI


Precipitator

Miniprep

Investigators: Sophie, Sandra

Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.

Miniprep of the plastik-binding domain:

samples DNA concentration 280/230 260/230
M2a alpha/M2a* alpha 66.5/91.6 1.84/1.75 1.76/1.40
M2a beta/M2a* beta 83.8/13.8 1.80/1.72 1.52/0.97
M2a gamma/M2a* gamma 118.9/56.1 1.68/1.86 1.00/2.13
M2a delta/M2a* delta 101.5/66.0 1.74/1.85 1.26/2.16
M2a epsilon/M2a* epsilon 112.9/69.9 1.68/1.82 1.00/1.82
M2b alpha/M2a* alpha 79.7/74.9 1.87/1.83 2.20/2.14
M2b Beta/M2b* beta 122.3/74.4 1.71/1.84 1.04/2.18
M2b gamma/M2b* gamma 111.2/72.0 1.70/1.83 1.08/1.99
M2b epsilon/M2b* epsilon 88.5/90.09 1.72/1.79 1.19/1.78
M3 alpha/M3* alpha 136.3/93.9 1.71/1.78 1.10/1.74
M3 beta/M3* beta 54.7/79.2 1.91/1.83 2.03/2.13
M3 gamma/M3* gamma 130.6/141.7 1.66/1.80 0.87/1.88
M3 delta/M3* delta 79.6/82.9 1.76/1.85 1.27/2.19
M3 epsilon/M3* epsilon 84.1/122.5 1.79/1.69 1.45/1.03
Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/13_July"