Team:Freiburg/Notebook/13 July
From 2011.igem.org
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+ | ===Testdigest=== | ||
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+ | '''Investigators: Julia''' | ||
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+ | Testdigest of cph8 | ||
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+ | *Enzyme 1: EcoRI | ||
+ | *Enzyme 2: PstI |
Revision as of 12:20, 15 July 2011
red light receptor
Investigators: Julia
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.
Miniprep
Investigators: Jakob
Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:
samples | DNA concentration | 260/280 | 230 |
cph8a | 33.7 | 1.79 | 1.74 |
cph8b | 41.2 | 1.79 | 1.39 |
cph8c | 24.1 | 1.87 | 1.47 |
cph8d | 30 | 1.74 | 1.84 |
cph8e | 34 | 1.81 | 1.86 |
cph8f | 30 | 1.83 | 1.81 |
Testdigest
Investigators: Julia
Testdigest of cph8
- Enzyme 1: EcoRI
- Enzyme 2: PstI