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Revision as of 10:26, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→Lysis cassette and heat induced promoter) ← Older edit		Revision as of 10:27, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→Green light receptor) Newer edit →
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	part is likely to be not effective		part is likely to be not effective
-	==Green light receptor==	+	==<span style="color:green;">green light receptor</span>==
	===CcaR===		===CcaR===
	S17: (CcaR with Prefix and RBS) is ok. Check RBS.		S17: (CcaR with Prefix and RBS) is ok. Check RBS.

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Revision as of 10:27, 15 July 2011

Contents 1 Sequence Analysis 1.1 Lysis cassette 1.1.1 Lysis cassette and heat inducible promotor 1.2 green light receptor 1.2.1 CcaR 1.2.2 pycA+terminator 1.2.3 cph8 1.2.4 LOV1 1.2.5 LOV3 2 Plastic binding site

Sequence Analysis

Lysis cassette

Lysis cassette and heat inducible promotor

S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective

green light receptor

CcaR

S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.

pycA+terminator

S24: prefix and suffix not complete

cph8

S29: Sequence without part. Has to be done again.

LOV1

S33: LOVtap only

LOV3

S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators.

Plastic binding site

Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15