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Team:Freiburg/Notebook/28 June
From 2011.igem.org
(→PCR of GFP fused to plastic-binding domain) |
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| + | ==<span style="color:green;">Digestion of minipreps for the green light receptor</span>== | ||
| + | |||
| + | '''Investigators: Sophie''' | ||
| + | |||
| + | Digestion of minipreps: | ||
| + | *S 17 (Ccar 1:1) | ||
| + | *S 18 (Ccar 1:3) | ||
| + | *S 19 (Ccas 1:1) | ||
| + | *S 20 (Ccas 1:3) | ||
| + | *S 21 (artificial terminator) | ||
| + | |||
| + | Enzyme 1: XbaI | ||
| + | Enzyme 2: PstI | ||
| + | |||
| + | two gels were loaded. | ||
| + | |||
| + | <span style="color:blue;">Comments: S17, S18 and S20 were ok. S21 was not digested.</span> | ||
| + | |||
| + | |||
| + | |||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
===3A assembly:Digestion, Ligation and Transformation for the receptor=== | ===3A assembly:Digestion, Ligation and Transformation for the receptor=== | ||
Revision as of 10:08, 15 July 2011
Contents |
Digestion of minipreps for the green light receptor
Investigators: Sophie
Digestion of minipreps:
- S 17 (Ccar 1:1)
- S 18 (Ccar 1:3)
- S 19 (Ccas 1:1)
- S 20 (Ccas 1:3)
- S 21 (artificial terminator)
Enzyme 1: XbaI Enzyme 2: PstI
two gels were loaded.
Comments: S17, S18 and S20 were ok. S21 was not digested.
Lysis cassette
3A assembly:Digestion, Ligation and Transformation for the receptor
Investigators: Julia
Digestion of ho1, pcyA (chromophor) and terminator (pSB1AK3).
Ho1 and pcyA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
Comments: The terminator between ho1 and pycA was missing. The digestion was repeated on june, 29.
Precipitator
PCR of GFP fused to plastic-binding domain
Investigators: Sophie, Rüdiger
S 14: GFP
Primer:
- GFPxb1Iup (P1)
- GFPpbdSpedw (P3)
Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30
Digestion of minipreps for the green light receptor
Investigators: Sophie
Digestion of minipreps:
- S 17 (Ccar 1:1)
- S 18 (Ccar 1:3)
- S 19 (Ccas 1:1)
- S 20 (Ccas 1:3)
- S 21 (artificial terminator)
Enzyme 1: XbaI Enzyme 2: PstI
two gels were loaded.
Comments: S17, S18 and S20 were ok. S21 was not digested.
