Team:Freiburg/Notebook/15 July

From 2011.igem.org

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(Meeting)
(Lysis cassette)
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attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra
attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra
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===<span style="color:green;">green light receptor</span>===
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already done:
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To-do:
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===<span style="color:orange;">Lysis cassette</span>===
===<span style="color:orange;">Lysis cassette</span>===

Revision as of 09:12, 15 July 2011

Contents

Meeting

attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra

green light receptor

already done:


To-do:



Lysis cassette

already done:

  1. repressor part (BBa_K098995) has no bases between RBS (B0034) and start codon (K098997), resulting in no translation.
    • quickchange of the repressor part to insert 6bp between RBS and start codon

To-do:

  1. DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand.
    • After digestion with DpnI, transformation of cells with the corrected part (BBa_K08995)

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME