Team:Freiburg/Safety
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+ | == '''Safety''' == | ||
- | + | We aim to transform protein expression in bacterial systems into an elegant, fast and affordable process. | |
+ | By eliminating routine use of expensive materials, this novel assay will utilize sustainable laboratory equipment and widespread His-Tag technology. | ||
- | + | We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of ''E. coli''. | |
+ | Purification of the his-tagged protein of interest will be accomplished by | ||
+ | an adaptor protein of our own design which binds the His-Tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product. | ||
- | + | Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-Tagged protein needed by the modern molecular biologist. | |
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Revision as of 13:21, 14 July 2011