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Revision as of 08:38, 13 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→Digestion of PR1-PR9) ← Older edit		Revision as of 12:14, 14 July 2011 (view source) Sandra.Wassner (Talk | contribs) m (→15. Labday) Newer edit →
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	==Lysis cassette test==		==Lysis cassette test==

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Revision as of 12:14, 14 July 2011

Lysis cassette test

Investigators: Theo

Comments: cells were used with the lysis cassette transformed on Saturday

5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed.

Digestion of PR1-PR9

Investigators: Julia, Theo

Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9.
Enzyme 1: XhoI
Enzyme 2: PstI
Samples were then loaded onto a gel.