"

From 2011.igem.org

Revision as of 16:56, 13 July 2011 (view source) EvaCar (Talk | contribs) (Created page with "==Wednesday==") ← Older edit		Latest revision as of 11:34, 14 July 2011 (view source) EvaCar (Talk | contribs)
(2 intermediate revisions not shown)
Line 1:		Line 1:
-	==Wednesday==	+	==Thursday==
		+
		+	Hurra - the two first edition A2 had the mutations. We'll have our second BioBrick very soon.
		+
		+	And the human cyps have grown marvellous - not like those lazy plant cyps. We are exhousted form making overnight cultures.
		+	===Lab Work===
		+
		+
		+	====Plant CYPs====
		+	'''BioBrick A1'''
		+
		+	*  We took the A1's out and put it in and overnight culture
		+
		+	'''BioBrick A2'''
		+
		+	* We extended our cyp A2 with prefix and suffix by a [[Team:Copenhagen/Protocol#PCR Reaction|PCR approach]].
		+
		+	* The DNA obtained was purified by using a [[Team:Copenhagen/Protocol#PCR_purification|PCR purification kit]].
		+
		+
		+	'''BioBrick B1'''
		+
		+	* The transformed bacteria had a cold night and needed more time to grow. So they spend the day in the 37 degress room.
		+
		+	====Human CYPs====
		+
		+	* Made overnight cultures.
		+
		+
		+
		+	Back to [[Team:Copenhagen/Notebook|Notebook]]

---

Latest revision as of 11:34, 14 July 2011

Contents 1 Thursday 1.1 Lab Work 1.1.1 Plant CYPs 1.1.2 Human CYPs

Thursday

Hurra - the two first edition A2 had the mutations. We'll have our second BioBrick very soon.

And the human cyps have grown marvellous - not like those lazy plant cyps. We are exhousted form making overnight cultures.

Lab Work

Plant CYPs

BioBrick A1

• We took the A1's out and put it in and overnight culture

BioBrick A2

• We extended our cyp A2 with prefix and suffix by a PCR approach.

• The DNA obtained was purified by using a PCR purification kit.

BioBrick B1

• The transformed bacteria had a cold night and needed more time to grow. So they spend the day in the 37 degress room.

Human CYPs

• Made overnight cultures.

Back to Notebook