Team:Freiburg/Notebook/4 July

From 2011.igem.org

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(Digestion of PCR products)
(Digestion of PCR products)
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<span style="color:blue;">Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.</Span>
<span style="color:blue;">Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.</Span>
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==Sequencing==
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'''Investigators: Theo'''

Revision as of 09:53, 14 July 2011

23. Labday


Digestion of PCR products

Investigators: Julia

Digest of PCR product Ccas.

Ccas:

  • Enzyme 1: XbaI
  • Enzyme 2: PstI

in vector pSB1C3:

  • Enzyme 1: SpeI
  • Enzyme 2: PstI

Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.


Sequencing

Investigators: Theo