Team:Peking S/lab/biosafety

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1. would any of your project ideas raise safety issues in terms of:
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•researcher safety,
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•public safety, or
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•environment safety?
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Since our project concentrates on developing a dynamic system based on bacterial cell-cell communication, there may be certain safety issues relating to researchers, the public and the environment regarding bacteria and gene parts. Therefore, a laboratory regulation had been established by team members according to national biosafety standards combined with requirements from experienced supervisors. It is required that researchers follow strict guidelines during experiments to minimize their exposure to potential risks. Bacteria gene parts are also carefully dealt with to prevent any possible gene transfer to the environment outside. Specifically, bacterial cells are preserved in certain conditions and all the materials and instruments, wastes included, are sterilized with alcohol or bleach after experiments. In addition, our protocols are carefully designed to eliminate potential hazards to the public or the environment.
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<div><font face="Castellar" class="ws22"><B>BIOSAFETY</B></font></div>
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<div><font face="Castellar" class="ws22"><B><BR></B></font></div>
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<div><font face="Castellar" class="ws22"><B><BR></B></font></div>
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<div><font face="Castellar" class="ws22"><BR></font></div>
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<div><font face="΢ÈíÑźÚ"><B>1. &nbsp; &nbsp;Would any of your project ideas raise safety issues in terms of:</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;researcher safety,&nbsp;</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;public safety, or&nbsp;</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;environment safety?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ">Since our project concentrates on developing a versatile and unique platform for bacterial cell-cell communication, there may beseveral safety issues relating to researchers, the public and the environment regarding the parts from pathogenic bacteria. Therefore, a laboratory regulation had been established according to national biosafety standards as well as with requirements from experienced supervisors. It is required that researchers follow strict guidelines during experiments to minimize their possible exposure to potential risks. Parts from pathogenic strains are also carefully dealt with to prevent any possible horizontal gene transfer to the environment outside. Specifically, bacterial cells are preserved in certain conditions and all the materials and instruments, wastes included, are sterilized with alcohol or bleach after experiments. In addition, our protocols are carefully designed to eliminate potential hazards to the public or to the environment.</font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ"><B>2. &nbsp; &nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</B></font></div>
 +
<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;did you document these issues in the Registry?&nbsp;</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;how did you manage to handle the safety issue?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;how could other teams learn from your experience?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B><BR></B></font></div>
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<div><font face="΢ÈíÑźÚ">No, none of our newly-made BioBrick parts would bring about any safety issues according to current professional information. &nbsp;Although our system includes several genes from Vibrio cholera, Streptomyces griseus and S. coelicolor, respectively, these genes are not directly involved with virulence or antibiotic resistance in any case.</font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ"><B>3. &nbsp; &nbsp;Is there a local biosafety group, committee, or review board at your institution?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;if yes, what does your local biosafety group think about your project?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B>&nbsp; &#8226;if no, which specific biosafety rules or guidelines do you have to consider in your country?</B></font></div>
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<div><font face="΢ÈíÑźÚ"><B><BR></B></font></div>
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<div><font face="΢ÈíÑźÚ">Yes. The project is supervised by several professors and managers in laboratory. The proposals, guidelines and protocols have been carefully revised regularly to ensure biosafety control.&nbsp;</font><font face="΢ÈíÑźÚ"><a href="  http://www.lab.pku.edu.cn/gzzdu.asp" title="">The Office of Laboratory and Equipment of Peking University</a></font><font face="΢ÈíÑźÚ">&nbsp;is responsible for any of our relevant safety issues.</font><font face="΢ÈíÑźÚ"><U>&nbsp;</U></font><font face="΢ÈíÑźÚ">This webpage provides links of laboratory management rules and regulations generated and implemented by the office,</font></div>
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<div><font face="΢ÈíÑźÚ">&nbsp;&nbsp;</font><font face="΢ÈíÑźÚ"><a href="http://www.lab.pku.edu.cn/gzzd/Lab_aqglbf.asp" title="">&nbsp;This page specially focuses on laboratory safety issues, biosafety included.</a></font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ">Also, we have taken national laws, rules, policies, standards and regulations into consideration. Our laboratory strictly follows the national standard &#8217; Laboratories&#8212;General requirements for biosafety&#8217;(GB19489-2008) and &#8216;Biosafety Regulation on Pathogen Microbes&#8217;.</font></div>
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<div><font face="΢ÈíÑźÚ">&nbsp; &nbsp;</font><font face="΢ÈíÑźÚ"><U>The links are as follows:</U></font></div>
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<div><font face="΢ÈíÑźÚ">&nbsp; &nbsp;</font><font face="΢ÈíÑźÚ"><a href="  http://gbread.sac.gov.cn/bzzyReadWebApp/standardresources.action?m=readFile&bzNum=GB 19489-2008&flag=1" title="">Laboratories&#8212;General requirements for biosafety</a></font></div>
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<div><font face="΢ÈíÑźÚ">&nbsp; &nbsp;</font><font face="΢ÈíÑźÚ"><a href=" http://www1.www.gov.cn/zwgk/2005-05/23/content_256.htm" title="">Biosafety regulation on pathogen microbes</a></font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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<div><font face="΢ÈíÑźÚ"><BR></font></div>
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</div></div>
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2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
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<div id="image1" style="position:absolute; overflow:hidden; left:682px; top:1370px; width:332px; height:223px; z-index:1"><img src="images/DSC_1337.JPG" alt="" title="" border=0 width=332 height=223></div>
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•did you document these issues in the Registry?
+
-
•how did you manage to handle the safety issue?
+
-
•how could other teams learn from your experience?
+
-
No, none of our newly-made BioBrick parts would bring about any safety issues according to current professional information.  Although our system includes several genes from Vibrio cholera, Streptomyces griseus and S. coelicolor, respectively, these genes are not directly involved with virulence in the case of V. cholera or antibiotics production in the case of Streptomyces species.
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<div id="image2" style="position:absolute; overflow:hidden; left:663px; top:501px; width:301px; height:174px; z-index:2"><img src="images/DSC_1360.JPG" alt="" title="" border=0 width=301 height=174></div>
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3. Is there a local biosafety group, committee, or review board at your institution?
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<div id="image3" style="position:absolute; overflow:hidden; left:683px; top:1624px; width:332px; height:234px; z-index:3"><a href="images/DSC_1456.JPG" target="_blank"><img src="images/DSC_1456.JPG" alt="" title="" border=0 width=332 height=234></a></div>
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•if yes, what does your local biosafety group think about your project?
+
-
•if no, which specific biosafety rules or guidelines do you have to consider in your country?
+
-
Yes. The project is supervised by several professors and managers in laboratory. The proposals, guidelines and protocols have been carefully revised regularly to ensure biosafety control. The Office of Laboratory and Equipment of Peking University is responsible for any of our relevant safety issues. This webpage provides links of laboratory management rules and regulations generated and implemented by the office,
+
<div id="text2" style="position:absolute; overflow:hidden; left:181px; top:1352px; width:498px; height:580px; z-index:4">
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    http://www.lab.pku.edu.cn/gzzdu.asp
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<div class="wpmd">
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    and this page specially focuses on laboratory safety issues, biosafety included.
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<div><font face="΢ÈíÑźÚ"><B>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</B></font></div>
-
    http://www.lab.pku.edu.cn/gzzd/Lab_aqglbf.asp
+
<div><font face="΢ÈíÑźÚ"><BR></font></div>
-
Also, we have taken national laws, rules, policies, standards and regulations into consideration. Our laboratory strictly follows the national standard ’ Laboratories—General requirements for biosafety’(GB19489-2008) and ‘Biosafety Regulation on Pathogen Microbes’.
+
<div><font face="΢ÈíÑźÚ">Biosafety issues are not only academic topics but also ethic and public affairs. Thus, public engagement is of necessity and significance. Here we are proposing the idea of an online safety workshop in both academic and plain styles. With the workshop pages teams or any other visitors may have an easy excess toward the most essential knowledge regarding researcher safety, public safety and environment safety as well as participate in the assessment of projects. &nbsp;Also, more new ideas about safety control might be collected through online forums. In addition, it is also a good idea to encourage safety issues to be discussed in human practice.</font></div>
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    The links are as follows:
+
<div><font face="΢ÈíÑźÚ">In order to minimize potential hazards through engineering, special attention should be placed on horizontal gene transfer (HGT). Engineered lab strains are less likely to compete with wild type strains in field niche and consequently HGT is the more possible occurrence for public or environmental safety issues.</font></div>
-
    Laboratories—General requirements for biosafety
+
<div><font face="΢ÈíÑźÚ">Since safety characteristics of a system may vary with its component gene parts, orthogonality and distribution of the system and self-destruction devices within single plasmids should be encouraged. By developing biologically orthogonal platform and distributing whole system into different membrane-insulated cells, HGT of any single gene would probably not give rise to negative effects of the system working together. As for the single gene case, a suicide system integrated in the standardized backbones may help. E.g. the plasmids could encode endogenous toxins, while engineered lab strains chromosomally bear anti-toxin gene. Toxin will work once the plasmid accidentally transformed, so that the hazards of HGT would be further reduced.</font></div>
-
    http://gbread.sac.gov.cn/bzzyReadWebApp/standardresources.action?m=readFile&bzNum=GB 19489-2008&flag=1
+
<div><font face="΢ÈíÑźÚ"><BR></font></div>
-
    Biosafety regulation on pathogen microbes http://www1.www.gov.cn/zwgk/2005-05/23/content_256.htm
+
</div></div>
-
4.  Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
+
</body>
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+
</html>
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Biosafety issues are not only academic topics but also ethic and public affairs, especially when it comes to public or environment safety. Thus, public engagement is of necessity and significance. Here we are proposing the idea of an online safety workshop in both academic and plain styles. With the workshop pages teams or any other visitors may have an easy excess toward the most essential knowledge regarding researcher safety, public safety and environment safety as well as participate in the assessment of projects.  Also, more new ideas about safety control might be collected through online forums. In addition, it is also a good idea to encourage safety issues to be discussed in human practice.
+
-
In order to minimize potential hazards through engineering, special attention should be placed on horizontal gene transfer(HGT). Engineered cells are less likely to compete with wild type strains in natural environments and consequently HGT is the more possible occurrence for public or environmental safety issues.
+
-
Since safety characteristics of a system may differ from its component gene parts, orthogonality and distribution of the system and self-destruction devices within single plasmids should be encouraged. By developing biologically orthogonal systems and distributing a whole system into different plasmids, HGT of any single gene part would not give rise to negative effects of the system working together. As for single gene parts, a suicide system integrated in the standardized backbones may help. E.g. the plasmids could contain certain intracellular toxins, while our strains of cells incubated in lab are integrated with anti-toxin coding sequences, so that the hazards of HGT would be further reduced.
+

Revision as of 09:00, 14 July 2011

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN"> Untitled

BIOSAFETY



1.    Would any of your project ideas raise safety issues in terms of:
  •researcher safety, 
  •public safety, or 
  •environment safety?

Since our project concentrates on developing a versatile and unique platform for bacterial cell-cell communication, there may beseveral safety issues relating to researchers, the public and the environment regarding the parts from pathogenic bacteria. Therefore, a laboratory regulation had been established according to national biosafety standards as well as with requirements from experienced supervisors. It is required that researchers follow strict guidelines during experiments to minimize their possible exposure to potential risks. Parts from pathogenic strains are also carefully dealt with to prevent any possible horizontal gene transfer to the environment outside. Specifically, bacterial cells are preserved in certain conditions and all the materials and instruments, wastes included, are sterilized with alcohol or bleach after experiments. In addition, our protocols are carefully designed to eliminate potential hazards to the public or to the environment.


2.    Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
  •did you document these issues in the Registry? 
  •how did you manage to handle the safety issue?
  •how could other teams learn from your experience?

No, none of our newly-made BioBrick parts would bring about any safety issues according to current professional information.  Although our system includes several genes from Vibrio cholera, Streptomyces griseus and S. coelicolor, respectively, these genes are not directly involved with virulence or antibiotic resistance in any case.

3.    Is there a local biosafety group, committee, or review board at your institution?
  •if yes, what does your local biosafety group think about your project?
  •if no, which specific biosafety rules or guidelines do you have to consider in your country?

Yes. The project is supervised by several professors and managers in laboratory. The proposals, guidelines and protocols have been carefully revised regularly to ensure biosafety control. The Office of Laboratory and Equipment of Peking University is responsible for any of our relevant safety issues. This webpage provides links of laboratory management rules and regulations generated and implemented by the office,

Also, we have taken national laws, rules, policies, standards and regulations into consideration. Our laboratory strictly follows the national standard ’ Laboratories—General requirements for biosafety’(GB19489-2008) and ‘Biosafety Regulation on Pathogen Microbes’.
   The links are as follows:


4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Biosafety issues are not only academic topics but also ethic and public affairs. Thus, public engagement is of necessity and significance. Here we are proposing the idea of an online safety workshop in both academic and plain styles. With the workshop pages teams or any other visitors may have an easy excess toward the most essential knowledge regarding researcher safety, public safety and environment safety as well as participate in the assessment of projects.  Also, more new ideas about safety control might be collected through online forums. In addition, it is also a good idea to encourage safety issues to be discussed in human practice.
In order to minimize potential hazards through engineering, special attention should be placed on horizontal gene transfer (HGT). Engineered lab strains are less likely to compete with wild type strains in field niche and consequently HGT is the more possible occurrence for public or environmental safety issues.
Since safety characteristics of a system may vary with its component gene parts, orthogonality and distribution of the system and self-destruction devices within single plasmids should be encouraged. By developing biologically orthogonal platform and distributing whole system into different membrane-insulated cells, HGT of any single gene would probably not give rise to negative effects of the system working together. As for the single gene case, a suicide system integrated in the standardized backbones may help. E.g. the plasmids could encode endogenous toxins, while engineered lab strains chromosomally bear anti-toxin gene. Toxin will work once the plasmid accidentally transformed, so that the hazards of HGT would be further reduced.

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