Sensor: Week 8 July 5-8
From 2011.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
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|Transformation/Plating | |Transformation/Plating | ||
- | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin | + | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin resistant plates. |
|} | |} | ||
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|J23113 + B0034 + C0051 | |J23113 + B0034 + C0051 | ||
- | |Good | + | |align="center"|Good |
|- | |- | ||
|KOR + K648000 | |KOR + K648000 | ||
- | |Good | + | |align="center"|Good |
|} | |} | ||
Revision as of 17:51, 13 July 2011
Tuesday, July 5
Clone OR and CRO
Protocol | Part Involved with Protocol | |
---|---|---|
Restriction Digest | Inserts using XbaI and PstI: | OR CRO |
Vector using XbaI and PstI: | K3 | |
Ligation | K3 + OR K3 + CRO | |
Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. |
cI Repressor Test Assembly, Day 27
Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:
Construct | Sequencing Result |
---|---|
J23113 + B0034 + C0051 | Good |
KOR + K648000 | Good |