Team:Freiburg/Notebook/28 June

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(Difference between revisions)
(3A assembly:Digestion, Ligation and Transformation for the receptor)
(PCR of GFP fused to plastic-binding domain)
Line 27: Line 27:
Primer: GFPxb1Iup  (P1)
Primer: GFPxb1Iup  (P1)
-
        GFPpbdSpedw (P3)
+
GFPpbdSpedw (P3)
<span style="color:blue;">Comments: P1 did not work</span>
<span style="color:blue;">Comments: P1 did not work</span>

Revision as of 13:22, 13 July 2011

21. Labday


3A assembly:Digestion, Ligation and Transformation for the receptor

Investigators: Julia

Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).

Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI

Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI

ligated vector was then transformed in cells and plated out on chloramphenicol plates.


PCR of GFP fused to plastic-binding domain

Investigators: Sophie, Rüdiger


S 14: GFP

Primer: GFPxb1Iup (P1) GFPpbdSpedw (P3)

Comments: P1 did not work