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Team:Freiburg/Notebook/28 June
From 2011.igem.org
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ligated vector was then transformed in cells and plated out on chloramphenicol plates. | ligated vector was then transformed in cells and plated out on chloramphenicol plates. | ||
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| + | ==PCR of GFP fused to plastic-binding domain== | ||
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| + | '''Investigators: Sophie, Rüdiger''' | ||
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| + | S 14: GFP | ||
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| + | Primer: GFPxb1Iup (P1) | ||
| + | GFPpbdSpedw (P3) | ||
| + | |||
| + | <span style="color:blue;">Comments: P1 did not work</span> | ||
Revision as of 13:21, 13 July 2011
21. Labday
3A assembly:Digestion, Ligation and Transformation for the receptor
Investigators: Julia
Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).
Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
PCR of GFP fused to plastic-binding domain
Investigators: Sophie, Rüdiger
S 14: GFP
Primer: GFPxb1Iup (P1)
GFPpbdSpedw (P3)
Comments: P1 did not work
