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Team:Freiburg/Notebook/28 June
From 2011.igem.org
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(→3A assembly:Digestion) |
(→3A assembly:Digestion and Ligation and Transformation) |
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| - | ==3A assembly:Digestion | + | ==3A assembly:Digestion, Ligation and Transformation for the receptor== |
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
| - | Digestion of ho1, pycA and terminator (pSB1AK3) | + | Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3). |
Ho1 and pycA:<br/> | Ho1 and pycA:<br/> | ||
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Enzyme 1: EcoRI<br/> | Enzyme 1: EcoRI<br/> | ||
Enzyme 2: XbaI<br/> | Enzyme 2: XbaI<br/> | ||
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| + | ligated vector was then transformed in cells and plated out on chloramphenicol plates. | ||
Revision as of 12:53, 13 July 2011
21. Labday
3A assembly:Digestion, Ligation and Transformation for the receptor
Investigators: Julia
Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).
Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
