Team:Freiburg/Notebook/28 June
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- | ==3A assembly:Digestion== | + | ==3A assembly:Digestion and Ligation and Transformation== |
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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Enzyme 1: EcoRI<br/> | Enzyme 1: EcoRI<br/> | ||
Enzyme 2: SpeI<br/> | Enzyme 2: SpeI<br/> | ||
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+ | Terminator part (pSB1AK3):<br/> | ||
+ | Enzyme 1: EcoRI<br/> | ||
+ | Enzyme 2: XbaI<br/> |
Revision as of 12:45, 13 July 2011
21. Labday
3A assembly:Digestion and Ligation and Transformation
Investigators: Julia
Digestion of ho1, pycA and terminator (pSB1AK3)
Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI