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Copenhagen/13 July 2011
From 2011.igem.org
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* We took the A1's out and put it in and overnight culture | * We took the A1's out and put it in and overnight culture | ||
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'''BioBrick A2''' | '''BioBrick A2''' | ||
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* We purified A2 (second edition) with [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] from 2 different colonies. | * We purified A2 (second edition) with [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] from 2 different colonies. | ||
* We analyzed A2 [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. Sent them both to be sequences. | * We analyzed A2 [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. Sent them both to be sequences. | ||
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'''BioBrick B1''' | '''BioBrick B1''' | ||
Revision as of 11:35, 13 July 2011
Contents |
Wednesday
Colonies on A1 - Finally. And our supervisors say that this is the easiest to work with. Hmm.
We still havent heard anything from our nice and helpful benefactors who supplied us with the human CYPs about the sequence and the composition of the plasmid. But we can't wayt any longer so we choose to believe that the plasmid contain ammphicilin. And then well get it sequenced - for this we will use the standard sequence of the middle of the CYPs which has most likely not been altered to much for expression in E.coli.
Lab Work
Plant CYPs
BioBrick A1
- We took the A1's out and put it in and overnight culture
BioBrick A2
- We purified A2 (second edition) with Mini prep from 2 different colonies.
- We analyzed A2 cut with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. Sent them both to be sequences.
BioBrick B1
Human CYPs
- Transformed XL1-Blue with human cyp 3A4, 2C9, 1A1, 1A2 and hNRP.
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