"
Copenhagen/4 July 2011
From 2011.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
===Lab Work=== | ===Lab Work=== | ||
| + | * On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture. | ||
| - | * We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. | + | * We checked our [[Team:Copenhagen/Protocol#Restrictionsite_analysis|restrictiondigest]] of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. |
* We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | ||
| Line 11: | Line 12: | ||
* Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | * Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | ||
| + | |||
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Latest revision as of 11:31, 13 July 2011
Monday
The rest of the team have returned, happy, well rested and eager to begin working.
Lab Work
- On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture.
- We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
- We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.
Afterwards we digested our mutants with Dnp - see protocols Mutations
- Transformed XL1-Blue competent cells - see protocol Transformations
Back to Notebook
