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Copenhagen/1 July 2011
From 2011.igem.org
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* We did a [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] of what we hope to be a plasmid containing CYP79B1 | * We did a [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] of what we hope to be a plasmid containing CYP79B1 | ||
| - | * We cut the (hopefully) purified CYP79A2 and CYP79B1 with restrictionsenzymes Pst1 and Xba1 to confirm that our mutations have destroyed these restrictionsites. | + | * We [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] the (hopefully) purified CYP79A2 and CYP79B1 with restrictionsenzymes Pst1 and Xba1 to confirm that our mutations have destroyed these restrictionsites. |
* We transformed competent cells with the mutants we made yesterday (with new and improved math) | * We transformed competent cells with the mutants we made yesterday (with new and improved math) | ||
* We made a lot of LB plates for future use. | * We made a lot of LB plates for future use. | ||
Latest revision as of 11:30, 13 July 2011
Friday
Wuuuhuuuuu! We have recieved our human cytochrome p450s with the mail today. What a relief. We are very grateful and send many happy thoughts to the sweet professor Guengerich who supplied them.
Lab Work
- We did a Mini prep of what we hope to be a plasmid containing CYP79B1
- We cut the (hopefully) purified CYP79A2 and CYP79B1 with restrictionsenzymes Pst1 and Xba1 to confirm that our mutations have destroyed these restrictionsites.
- We transformed competent cells with the mutants we made yesterday (with new and improved math)
- We made a lot of LB plates for future use.
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